Ten small ponds (New, Lizard Pen, Salamander, E. Twin, W. Twin, Railroad, Teardrop, South, Hidden and Well) have been constructed or developed at Tyson since 1963. In addition, there are three retired sewage lagoons in the laboratory area which are utilized by wildlife. These water bodies serve as focal points for animal activities and for continuing studies of salamanders and other amphibians.
In addition, one permanent spring, Blue Grass Spring, and an additional 10-15 intermittent springs add to the diversity of aquatic habitats available. Blue Grass is the largest of these and produces Tyson's only permanent stream. Since the spring is only 100m from the south fence, the length of stream habitat available is correspondingly limited.
Tyson also borders a large bend in the Meramec River across from Castlewood State Park. This portion of the river carries more sediment and is wider than its upper reaches; however, it is still very valuable for scientific study.